Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants
نویسندگان
چکیده
منابع مشابه
Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation
Amniotic fluid (AF) was described as a potential source of mesenchymal stem cells (MSCs) for biomedicine purposes. Therefore, evaluation of alternative cryoprotectants and freezing protocols capable to maintain the viability and stemness of these cells after cooling is still needed. AF stem cells (AFSCs) were tested for different freezing methods and cryoprotectants. Cell viability, gene expres...
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THREE MODES FOR CRYOPRESERVATION (CP) OF HUMAN IPSC CELLS HAVE BEEN COMPARED: STD: standard CP of small clumps with 10% of CPA in cryovials, ACC: dissociation of the cells with Accutase and freezing in cryovials, and PLT: programmed freezing of adherent cells in plastic multiwell dishes in a programmable freezer using one- and multistep cooling protocols. Four CPAs were tesetd: dimethyl sulfoxi...
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However, early efforts to incorporate AI into production schemes were limited by the functional lifespan of ejaculated spermatozoa. Therefore, widespread the use of AI Cryopreservation (CP) is the ability to store cells and maintain their integrity and viability at a sub-zero temperature until needed. Like some of the greatest discoveries, CP of semen was the result of a fortunate lab error. In...
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Background Cryopreservation procedure for poultry semen is not reliable due to structural and biochemical damages which can lead to reduction in sperm quality and fertility. Development of cryopreservation medium has crucial effects for successful recovery of sperm after freezing. The objective of this study was assessment of cryopreservation of rooster semen using hand-made cryopreservation me...
متن کاملComparison of cryoprotectants and methods of cryopreservation of fowl spermatozoa.
The deleterious effects of three cryoprotectants, glycerol, dimethylsulfoxide (DMSO), and dimethylacetamide (DMA), were compared on fowl spermatozoa. The viability and integrity of spermatozoa were measured with eosin-nigrosin smears. Glycerol was the least deleterious cryoprotectant, followed by DMA, and DMSO was the most toxic. Methods employing either glycerol or DMA were then compared for t...
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ژورنال
عنوان ژورنال: ACS Applied Bio Materials
سال: 2020
ISSN: 2576-6422,2576-6422
DOI: 10.1021/acsabm.0c00638